#Japan #Tokyo #Skin Barrier #Yuzu Extract #TRPV4 Channel

The Groundbreaking Study on Yuzu Extract

In an innovative research breakthrough, the POLA Innovation Center, in collaboration with Professor Takeshi Matsui from Tokyo University of Technology, is set to present significant findings at the 50th Annual Meeting of the Japanese Society of Cosmetology. The study focuses on the extract derived from yuzu pomace, which has shown promising effects on human keratinocytes, particularly emphasizing its ability to increase intracellular calcium ion concentrations (Ca2+).

Study Highlights

Title of the Paper

"Utilization of Unexploited Resources: Yuzu Pomace Extract Promotes Intracellular Ca2+ Concentration Rise"

Researchers Involved

• - Naoko Sato, Takeshi Matsui - Tokyo University of Technology, Faculty of Applied Biological Sciences
• - Akihiro Tada - POLA Innovation Center

Key Findings

1. Calcium Ion Concentration Increase
2. TRPV4 Channel Mediated Ca2+ Influx

Through advanced imaging techniques, the research investigates how yuzu extract affects calcium concentrations in human primary cultured keratinocytes. By introducing a calcium probe, Rhod-4, AM, the researchers monitored changes in Ca2+ levels in cells treated with the yuzu extract, alongside TRPV4 inhibitors or activators. The extraction process utilized a 50% ethanol aqueous solution with pomace sourced from Kochi Prefecture.

Calcium Ion Concentration Increase

Upon application of the yuzu pomace extract, a notable increase in intracellular Ca2+ levels was observed across many cells. This suggests that the extract activates calcium channels, as evidenced by the rise in Ca2+ levels indicated by magenta arrows in the accompanying figures.

TRPV4 Channel Mediated Ca2+ Influx

The study highlighted that when TRPV4 inhibitors were used, the increased Ca2+ levels prompted by the yuzu extract were suppressed, indicating that TRPV4 activation is crucial for Ca2+ influx. Conversely, when a TRPV4 activator was included, a pronounced increase in intracellular Ca2+ was recorded, further underscoring TRPV4's role in this process.

It became evident that TRPV4's activation is integral to the functionality of tight junctions in the epidermis, which serve as a protective barrier for the skin. Specifically, when TRPV4 is in an activated state, it aids in the formation of tight junctions, thus enhancing skin barrier functions.

Practical Implications

The implications of these findings are tremendous, especially for those seeking solutions for skin health during winter months when dryness poses significant challenges. The study suggests that yuzu extract could enhance skin barrier function by activating TRPV4, promoting tight junction formation crucial for skin hydration and health.

The correlation between temperature and TRPV4 activation indicates that maintaining a warm environment could further amplify these protective effects. Therefore, yuzu-derived products may not only be beneficial during colder seasons but could revolutionize how we approach skincare in general.

Conclusion

In conclusion, the extraction of yuzu pomace offers a novel natural resource with the potential to enhance skin health through its effects on intracellular calcium levels and the activation of critical channels such as TRPV4. As we anticipate deeper insights during the conference, this research indicates a promising future for yuzu in skincare science and product development.