#United States #New England Biolabs #Ipswich #NEBNext #RNA Library Kit

New England Biolabs Launches NEBNext Low-bias Small RNA Library Prep Kit

In a groundbreaking development for molecular biology research, New England Biolabs (NEB) has officially launched the NEBNext Low-bias Small RNA Library Prep Kit. This innovative kit is designed to address long-standing issues of bias in small RNA sequencing, enabling researchers to capture a more accurate representation of the diversity inherent in RNA samples. As reiterated by Betsy Young, Ph.D., Senior Product Marketing Manager for the NEBNext portfolio, this new offering empowers scientists to explore the roles of small RNAs, which have been overlooked in many studies due to their subtle yet significant impacts on health and disease.

Understanding the Challenge

Historically, small RNAs, including rare and non-coding varieties, have posed considerable challenges in RNA sequencing. Their small sizes often lead to significant sequencing biases, meaning that certain small RNAs are preferentially represented while others remain undetected. Such limitations have hindered the comprehensive understanding of RNA diversity and abundance in biological samples. The NEBNext Low-bias kit aims to resolve these biases through a fresh approach that minimizes variability in ligation efficiency—one of the primary culprits behind sequencing discrepancies.

Key Features of the NEBNext Kit

The NEBNext Low-bias Small RNA Library Prep Kit showcases several important advancements:
1. Protocol Enhancements: The kit integrates a novel splint adaptor, which enhances ligation efficiency across a broader diversity of RNA interactions. This improvement increases sensitivity, allowing for the effective analysis of various RNA species present in samples.
2. Unprecedented Speed: Researchers can now complete RNA library preparations within approximately 3.5 hours, significantly reducing the time from sample to results.
3. Extended Shelf Life: The kit boasts an impressive 18-month shelf life, ensuring researchers can utilize it over a longer duration without compromising efficacy.
4. Versatile Input Range: This kit accommodates both standard and 2′-O-methylated samples, broadening its applicability. Multiplexing is enabled with up to 480 compatible UDI primer pairs, which can be purchased separately.

The development of this groundbreaking kit was rooted in over a decade of meticulous research by NEB scientists. Notably, foundational studies from 2012 and 2020 identified the critical issues surrounding RNA sequencing biases and introduced improved methods to enhance sensitivity and reduce bias.

A Surge in RNA Research Opportunities

As Greg Lohman, Research Group Leader in NEB's Molecular Enzymology Division, highlights, the NEBNext Low-bias kit is poised to revolutionize small RNA research. By providing a tool that effectively tackles bias-related challenges, it paves the way for researchers to uncover new insights into the role of RNA molecules in various biological contexts. This is expected to significantly ease the complexities associated with RNA sequencing data interpretation and expand the horizons of RNA research.

Moving Forward

For researchers eager to incorporate this innovative technology into their work, further information and trial kit requests can be initiated through the NEB website at NEBNext Low-bias Small RNA Library Prep Kit page.

About New England Biolabs

New England Biolabs has been at the forefront of molecular biology research for over 50 years, continually advancing the field with innovative and high-quality products. NEB remains dedicated to scientific discovery, reflected in their expansive product portfolio and commitment to supporting both academia and the biotechnology industry. Headquartered in Ipswich, Massachusetts, NEB operates globally, fueled by a promise to enhance the well-being of their employees, communities, and the planet at large.

With the launch of the NEBNext Low-bias Small RNA Library Prep Kit, NEB is setting a new benchmark in RNA research, promising researchers the tools needed to delve deeper into the complexities of RNA biology.