KROMATID Unveils Next-Gen KROMASURE™ PinPoint for Transgene Integration Analysis

KROMATID Revolutionizes Transgene Integration Analysis with KROMASURE™ PinPoint

KROMATID, a trailblazer in the field of structural genomics, has recently launched an innovative solution aimed at advancing transgene integration analysis, the KROMASURE™ PinPoint. This advanced platform represents a significant leap forward in the realm of genomic research, providing researchers with unprecedented capabilities to analyze gene editing processes at a single-cell resolution.

A New Era for Genetic Research

The KROMASURE™ PinPoint solution introduces a proprietary high-throughput approach that sets a new standard for the analysis of transgene genomic integration. Researchers can now detect integration events as small as 2 kilobases (kb) and analyze up to 1,000 individual cells in one go. This groundbreaking capability enhances the quality of data researchers can gather, offering insights that were previously unattainable using conventional pooled assays.

According to Erin Cross, Vice President of the Platform at KROMATID, “PinPoint is not just a product—it's a transformation in how we visualize and understand transgene insertion.” The KROMASURE™ PinPoint's design not only allows for high-resolution analysis, but it is also optimized for cell and gene therapy development—an area that's rapidly evolving in biotechnology.

Enhanced Insights and Detectability

One of the most significant advantages of KROMASURE™ PinPoint is its ability to provide precise, quantitative insights into transgene insertional copy number and its distribution on a single-cell basis. Traditional methods of analysis such as sequencing and ddPCR, while effective, have limitations. They usually rely on pooled cell populations which tend to obscure crucial cell-to-cell variability. This means that low-prevalence events, which could be vital in understanding gene therapy outcomes, often go undetected.

With the advent of PinPoint, researchers are now empowered to visualize and profile transgene integrations across thousands of single cells. This expanded level of analysis not only heightens the accuracy of gene integration studies but also equips developers with a clearer picture of their editing mechanisms.

Cross further explains, “This expanded capability gives developers a clearer picture of how their editing machinery performs at the cellular level, helping them understand transgene distribution with far greater precision than pooled averages can provide.”

Breaking Ground with Advanced Data Insights

KROMATID's KROMASURE™ PinPoint paves the way for advanced data interpretation by delivering precise chromosomal mapping, effectively pinpointing exact integration loci down to individual chromosomes. This high-throughput analysis enables categorization of rare, low-prevalence events, facilitating data-driven safety assessments vital for regulatory compliance and risk profiling processes in gene therapy and cell line development.

Additionally, the interphase visualization feature allows for the detection of integrations in non-dividing cells—a capability that expands the analytical horizon beyond conventional cytogenetic methods that typically focus on dividing cells.

KROMASURE™ PinPoint integrates seamlessly with KROMASURE™ InSite for a comprehensive genomic insight, allowing for orthogonal validation that supports the thoroughness of data collection and analysis.

KROMATID's Commitment to Innovation

KROMATID serves as a contract research organization (CRO) that specializes in cytogenetic precision, offering advanced genomic integrity analysis solutions. The launch of KROMASURE™ PinPoint exemplifies KROMATID's commitment to enhancing the capabilities of researchers in the cell and gene therapy spaces, thereby accelerating breakthroughs in genomic medicine. As this technology continues to develop, KROMATID aims to address the critical gaps left behind by traditional sequencing methods while fostering a landscape of data-driven innovation.

For further information on KROMATID and the KROMASURE™ platform, please visit www.kromatid.com.