#China #Beijing #Tsingke Biotech #AI Protein Design #High-Throughput Validation

Tsingke's Innovative Approach to Protein and Antibody Validation

In a remarkable advancement for biotechnology, Tsingke Biotech has introduced a sophisticated high-throughput validation workflow aimed at revolutionizing the way researchers evaluate AI-designed proteins and antibodies. This cutting-edge framework combines various stages of gene synthesis, expression, screening, and binding assays to enable researchers to explore vast libraries of computationally generated candidates swiftly.

The Role of AI in Protein Design

The integration of artificial intelligence into protein and antibody design is a game-changer that accelerates how quickly researchers can generate viable candidates. Nan Zhang, Marketing Manager at Tsingke, emphasizes that while AI expedites the design process, there remains a crucial challenge: translating these designs into meaningful experimental data. Tsingke's high-throughput validation addresses this by allowing for large-scale screening and selection of the most promising candidates that can be characterized further.

Staged Validation Process

Tsingke's workflow is structured into distinct stages that facilitate an efficient validation process:

1. High-Throughput Candidate Generation and Screening: The journey begins with batch gene synthesis, followed by simultaneous expression of proteins or antibodies. This approach allows researchers to test a large set of candidates at once. Initial screening utilizing methods like ELISA facilitates the identification of candidates exhibiting desired characteristics, including binding efficiency.

2. Focused Prioritization of Selected Candidates: Once the initial pool has been narrowed down, prioritized candidates proceed to scale-up expression and purification. This allows for gram-scale production, which is crucial for conducting detailed quantitative binding analyses using sophisticated techniques such as BLI (Biolayer Interferometry) or SPR (Surface Plasmon Resonance). These tests yield essential kinetic data such as binding affinity and release rates, further informing researchers about each candidate's potential.

Robust Capabilities for Large-Scale Validation

Tsingke's high-throughput approach is bolstered by its expansive capabilities across the different facets of protein synthesis and expression. With expertise in handling complex sequences, the company can synthesize DNA fragments up to 200 kb, and manage constructs with challenging features such as high GC content or tandem repeats. The proprietary GeneOptimizer algorithm optimizes codon sequences for better compatibility with expression systems.

Diverse Expression Platforms

To ensure that various protein characteristics are met, Tsingke provides multiple expression systems, including mammalian cell lines and cell-free platforms. This flexibility allows researchers to select the expression strategy that best aligns with their specific project requirements. Additionally, Tsingke offers a range of antibody formats such as scFv, VHH, Fab, and full-length IgG, processing up to 1,500 candidates daily with quick turnaround times of around 12 days, including shipping.

Supporting Researchers Globally

Tsingke has been at the forefront of supporting innovative projects involving AI-generated proteins and antibodies. As the demand for efficient experimental validation of these molecules grows, the company is committed to enhancing not only its technical capabilities but also its service offerings, facilitating a smoother transition of designs from computational to experimental realms.

About Tsingke

Founded in Beijing, Tsingke Biotech Co., Ltd. specializes in providing comprehensive solutions that advance research and therapeutic development. With a foundation built on molecular biology, genetics, and protein science, Tsingke offers a plethora of services such as custom DNA/RNA synthesis, gene cloning, protein expression, and antibody discovery. Armed with ISO 13485-certified facilities and a technologically advanced approach, Tsingke enables innovation in drug discovery and genetic engineering, empowering researchers worldwide with state-of-the-art biomanufacturing capabilities.

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