#USA #New England Biolabs #Ipswich #Molecular Biology #Flu A Virus

New England Biolabs® Launches NEBNext® Flu A Integrated Indexing Primer Module

On November 13, 2025, New England Biolabs (NEB®) unveiled a groundbreaking product designed to enhance the surveillance and sequencing of Influenza A viruses. The NEBNext Flu A Integrated Indexing Primer Module (NEB #E3436) not only streamlines the sequencing process but also accommodates various sample types using the Oxford Nanopore Technologies (ONT) platforms.

A Leap in Influenza A Monitoring

This newly launched module represents a significant advancement in the fight against seasonal and emerging influenza subtypes, including strains like H3N2, H1N1, and H5N1. Co-developed with the Johns Hopkins Applied Physics Laboratory (APL), the NEBNext module integrates a multi-segment RT-PCR workflow with innovative indexing capabilities, which facilitate the sequencing of up to 48 samples in a single run.

The ability to effectively monitor these viruses is crucial as they can pose severe risks to public health. Peter Thielen, a molecular biologist at APL, emphasized the importance of rapid genetic characterization methods. He stated, "To effectively limit the spread of emerging biological threats such as highly pathogenic avian influenza, we need methods that are as quick and simple as diagnostic assays."

Impact on Public Health

The iiMS-PCR (integrated indexing multi-segment RT-PCR) workflow enables rapid responses to viral outbreaks, which is paramount for timely public health actions. Dr. Erik Karlsson, Head of the Virology Unit at Institut Pasteur du Cambodge, highlighted that the workflow had been crucial in responding to H5 outbreaks. With the ability to confirm poultry-to-human transmission within 24 hours, he stressed how critical it is for public health communication and action.

Moreover, the NEBNext module is designed to work in various settings, including public health laboratories and academic research, along with wastewater-based epidemiology programs. This versatility ensures that surveillance can happen wherever it's needed most, particularly in low-resource settings.

Comprehensive Components of the Module

The NEBNext Flu A Integrated Indexing Primer Module comes equipped with essential reagents for indexed cDNA synthesis, amplification, and library preparation. It operates in harmony with NEB’s Monarch® Mag Viral DNA/RNA Extraction Kit and LunaScript® Multiplex One-Step RT-PCR Kit. Additionally, the protocol for this primer module was proactively shared on protocols.io, ensuring that users gain early access to its beneficial workflow and confirming its effectiveness across various sample types.

NEB's Commitment to Global Health

Betsy Young, Ph.D., Senior Product Marketing Manager for Next Generation Sequencing at NEB, stated, "Influenza A poses an ongoing global health challenge due to its rapid evolution and seasonal variability. This new primer module empowers researchers and surveillance teams with a reliable workflow to monitor genetic changes and allocate public health resources adeptly."

The introduction of this module expands NEB's extensive portfolio, which already supports infectious disease research with offerings for SARS-CoV-2 and other respiratory pathogens.

For those interested in learning more, further information can be found on NEB's website at www.neb.com/E3436.

About New England Biolabs

For over five decades, New England Biolabs has been at the forefront of discovery and production of innovative products for molecular biology research. Committed to advancing scientific discovery, NEB continuously expands its product offerings and invests in research initiatives, ensuring that cutting-edge technologies are accessible in both academic and industrial applications. The company is based in Ipswich, Massachusetts, and operates globally through its subsidiaries and distribution partners.

For more detailed insights into their product innovations and public health initiatives, visit www.neb.com.