#Japan #Shibuya #Eternam #Juntendo University #MSC Culture Medium

Exploring the Potential of MSC Culture Medium in Anti-Aging

In recent years, interest in regenerative medicine has surged, with a particular focus on the therapeutic potential of stem cells. Among these advancements, research into the culture medium derived from human umbilical cord stem cells has been gaining attention for its potential applications in anti-aging and skin rejuvenation. A collaborative study between Eternam Inc. and Juntendo University has recently brought new insights into this area, culminating in a significant oral presentation at the 44th Japanese Society of Aesthetic Dermatology held on August 1, 2026.

The Research Focus: Understanding MSC Culture Medium

The study concentrated on the effects of the culture medium from human umbilical cord-derived mesenchymal stem cells (UCMSC-CM) on skin cells, specifically focusing on its potential to inhibit aging. UCMSC-CM contains a plethora of beneficial components, such as growth factors, cytokines, chemokines, exosomes, and microRNAs. These substances are thought to contribute to skin regeneration, anti-inflammatory responses, tissue repair, and overall anti-aging effects. Despite its promising potential, much remained to be explored regarding how UCMSC-CM influences gene expression in human keratinocytes and how it aids in controlling the aging process.

Groundbreaking Results on Aging Factors

The research employed several methodologies to evaluate the protective effects of UCMSC-CM against aging factors. One standout finding included the UCMSC-CM's ability to suppress the expression of critical markers associated with cellular aging, such as p21 and p16, when human epidermal keratinocytes were exposed to stimulating agents like hydrogen peroxide (H₂O₂) or UVA irradiation.

Data Insights

• - Cell Cycle Regulation: The introduction of UCMSC-CM maintained p21 levels comparable to unstimulated cells despite H₂O₂ and UVA challenges, suggesting a robust protective role in cellular proliferation.
• - β-Galactosidase Accumulation: Utilizing the SPiDER-β-Gal detection kit, a notable 7% reduction in β-galactosidase levels was observed, indicating a potential for reversing age-related cellular markers.

In addition, researchers assessed DNA damage through γH2AX immunostaining. A decrease in γH2AX-positive cells indicated successful inhibition of oxidative DNA damage, showcasing the anti-oxidative capabilities of UCMSC-CM.

Comprehensive Gene Expression Analysis

The impact of UCMSC-CM on gene expression was further elucidated through RNA sequencing of keratinocytes derived from different age groups—newborns, adolescents, and adults. The results highlighted a significant upregulation of genes associated with tissue repair and cell proliferation in both young and adult-derived keratinocytes, particularly in the adult group.

This suggests that UCMSC-CM could provide crucial support in promoting cellular regeneration and healing, especially in aging skin.

Conclusion and Future Directions

The findings of this comprehensive research underline the remarkable benefits of UCMSC-CM in mitigating aging markers and oxidative stress. The pronounced increase in gene expression related to tissue repair and cell growth in the adult epidermal cells points toward exciting possibilities for future applications in skincare and regenerative therapies.

Eternam Inc. aims to continue contributing to the field of skincare and regenerative medicine, utilizing the findings from this research to confront the growing complexities surrounding age and beauty. The study reinforces the mission of Eternam: to create a future where the dream of eternal beauty does not end in mere aspiration.

About Eternam

Eternam is a skincare brand born from regenerative medicine research. The name 'Eternam' derives from the Latin word for 'eternal,' symbolizing its commitment to innovative skincare solutions based on the proprietary extraction of mesenchymal stem cell culture medium. With products aimed at consumers and exclusive medical institutions, Eternam strives to deliver unmatched beauty and wellness, deeply resonating with the aspiration for eternal beauty.