RNAConnect, a pioneering name in life sciences, has unveiled its latest innovation—the UltraMarathonRT® Direct RNA-Seq Kit. This cutting-edge solution is designed for native RNA library preparation specifically for Oxford Nanopore Technologies® direct sequencing (SQK-RNA004). Unlike previous methods, which often fall short in detecting longer transcripts and complex gene variants, the UltraMarathonRT® kit opens new avenues for researchers by enhancing the representation of long RNA transcripts and providing more comprehensive data.
Optimized Workflow for Enhanced Results
The UltraMarathonRT Kit employs a groundbreaking approach during the reverse transcription (RT) phase, maintaining a gentle 30°C stabilization step that preserves RNA integrity. This low-temperature process is crucial for retaining endogenous RNA modification signals, ensuring that researchers can obtain reliable and biologically relevant data. By integrating this kit into their existing experiments, scientists can now expect:
- - 25-50% higher cDNA yield.
- - 5-10% longer read lengths, particularly beneficial for transcripts exceeding 2kb.
- - 1-2% more genes identified in their analyses.
- - 15-20% increase in novel transcripts detected, indicating improved sensitivity.
- - 25-30% boost in intron retention events observed.
These enhancements translate to richer insights into gene expression dynamics, particularly for complex biological systems where alternative splicing and functional diversity have critical implications.
Ease of Use with Proven Performance
Dr. Jason Underwood, RNAConnect's VP of Technology Development, stated, "We have carefully analyzed existing direct RNA-sequencing protocols to identify opportunities for improving long-read outputs. The UltraMarathonRT's unique properties align perfectly with our knowledge, allowing us to elevate RNA sequencing to new heights." This sentiment is echoed by Dr. Paul Boutz, Assistant Professor at the University of Rochester, who noted how the UltraMarathonRT Kit has revolutionized their ability to explore alternative splicing, a key area for many molecular studies.
Moreover, the user-friendly design of the kit ensures that both novice and experienced researchers can efficiently incorporate it into their workflows without extensive retraining. This accessibility aligns with RNAConnect’s mission to empower scientists across various fields—including rare diseases, oncology, and neurobiology—by providing them with robust tools for RNA analysis.
Availability and More Information
The UltraMarathonRT® Direct RNA-Seq Kit is now readily available for purchase through RNAConnect's website and through authorized distributors. For those interested in delving deeper into the specifications, compatibility, or for guidance on implementing this innovative tool in their research, RNAConnect invites inquiries to assist in maximizing the benefits of this new kit.
About RNAConnect
RNAConnect stands at the forefront of RNA research tools, dedicated to providing innovative solutions that enrich the study of genomics, transcriptomics, and molecular diagnostics. By continuously advancing their product offerings, RNAConnect ensures that researchers are equipped with accurate, reliable, and sensitive tools for their discoveries.
For media inquiries or to learn more about RNAConnect’s offerings, please contact:
Andrew Bond, PhD — Chief Commercial Officer
Tel: +1‑475-338-2120
Email: [email protected]
This launch marks a significant milestone in the evolution of RNA sequencing technologies and will likely pave the way for groundbreaking discoveries in RNA biology.