Volta Labs Launches an Automated Solution for Library Preparation for Oxford Nanopore's Ligation Sequencing Kit V14

Volta Labs Reveals Automated Library Preparation Solution for Oxford Nanopore V14

Volta Labs, known for its innovative Callisto™ sample preparation system, has announced the launch of a new application designed specifically for DNA library preparation using Oxford Nanopore Technologies' ligation sequencing kit V14 (SQK-LSK114). This cutting-edge solution enables laboratories to achieve fully automated library preparation, greatly enhancing efficiency and accuracy in genomic research.

Transforming DNA Library Preparation

With the new Callisto™ library preparation protocol, which is part of Oxford Nanopore’s compatible products program, labs can automate the library preparation process for the PromethION platform. What distinguishes this application is its ability to handle high molecular weight (HMW) DNA, which has been a challenge in many traditional workflows.

A comparative study and independent clinical assessment conducted at the University Medical Center Utrecht (UMC Utrecht) showcased the capabilities of libraries prepared with the Callisto system. During testing, researchers reported achieving an impressive output of up to 120 gigabases (Gb) of data from a single PromethION flow cell. This outcome not only highlighted the system's ability to generate substantial data but also its exceptional variant calling precision. The study noted a remarkable SNV recall rate of 99.66%, underscoring the system's reliability in producing diagnostic-quality results.

As Bert van der Zwaag from the Genetics Department at UMC Utrecht stated, “In evaluating whole genome sequencing for routine clinical diagnostics, having a robust and automated workflow is essential. The HMW DNA extraction and library preparation from Callisto gave us the consistency and throughput needed to progress towards ISO 15189 accreditation for Oxford Nanopore-based sequencing while delivering a variant calling accuracy comparable to our established short-read pipelines.”

Benefits of the Callisto System

Callisto™ not only simplifies the library preparation process but also enables laboratories to maintain high standards of accuracy and efficiency. Thomas Bray, Vice President of Corporate Business Development at Oxford Nanopore Technologies, added, “The results demonstrated with libraries prepared using the Callisto sample preparation system show the performance that our platform can achieve when paired with high-quality sample preparation for whole genome sequencing applications. We are excited to welcome Volta Labs into our compatible products program.”

Innovation at Its Core

Founded in 2018 at MIT, Volta Labs has been at the forefront of genomic sample preparation innovation. The Callisto™ system leverages proprietary electrowetting technology to provide fully automated processes for DNA and RNA extraction, with library preparation compatible with major sequencing platforms including Illumina, Oxford Nanopore, PacBio, Element Biosciences, and Ultima Genomics. This technology not only reduces intervention time by up to 80% but also offers unmatched reliability for a variety of sample types and chemical compositions.

Located in Boston, Massachusetts, Volta Labs is committed to transforming how genomic samples are prepared, making significant strides toward improving the accessibility and accuracy of genomic research. For further details, visit voltalabs.com.