Tethis Introduces SmartBioSurface® Slides for Enhanced Cell Imaging Workflows
Tethis S.p.A., a pioneering company in the life sciences and diagnostics sector, has recently unveiled an application note concerning its innovative SmartBioSurface® slides. These slides are specifically crafted for optimizing cell suspension workflows on leading spatial biology platforms, thus addressing a significant challenge faced in multi-cycle imaging: the stability of suspension-derived, non-adherent cells.
The focus on cell adhesion has become increasingly critical, especially when it comes to the analysis of suspension-derived samples such as Peripheral Blood Mononuclear Cells (PBMCs) and circulating tumor cells. Conventional imaging methods often struggle to maintain the integrity of these cells during repeated cycles of washing, staining, and imaging due to their lack of inherent anchorage to sample carriers. Tethis' SmartBioSurface slides are coated with a unique nanostructured titanium dioxide layer that mimics the extracellular matrix, significantly enhancing the immobilization of non-adherent cells while preserving their assay performance.
The application note showcases a rigorous study conducted using cryopreserved human PBMCs as representative samples. The findings from the study highlight several impressive outcomes:
1. Enhanced Cell Attachment: The initial attachment of PBMCs to the SmartBioSurface slides was more than double that of standard charged glass controls. This suggests that Tethis' innovative technology can provide a more effective platform for cell analysis.
2. Minimal Cell Loss: Throughout the multi-cycle imaging process, the observed cell loss averaged only about 0.14% per cycle. Remarkably, after ten imaging cycles, approximately 98.5% of cells remained intact. Additionally, no cell loss was detected after two weeks of storage at 4°C following fixation, demonstrating the slides' capacity for long-term sample stability.
3. Consistent Marker Performance: The slides exhibited reliable performance across a complex 15-plex immune panel, with all markers surpassing the established signal-to-noise threshold necessary for dependable detection and classification.
Given that samples derived from suspensions play an increasingly crucial role in spatial tissue profiling and liquid biopsies, Tethis’ SmartBioSurface slides are set to become a game-changer. They provide a stable two-dimensional layer for capturing suspension-derived cells, making them instrumental in advancing high-plex analysis of these cells, which can often be challenging due to their non-adherent nature. Tethis CEO Holger Neecke emphasized that this gentle adhesion technique plays a vital role in preserving cellular morphology and the intricate structures of suspension cells. This breakthrough paves the way for enhanced studies on rare cells and intricate immune-cell interactions, potentially unlocking new insights into various health conditions and treatments.
Furthermore, the application note characterizes SmartBioSurface slides as a scalable solution for sample preparation in high-plex biomarker profiling of suspension-derived cells. The implications of this technology are vast, with promising applications in immuno-oncology, infectious disease research, and innovative translational studies. For researchers interested in utilizing these advancements, Tethis has made the application note available for download at tethis-lab.com/smartbiosurface.
About Tethis S.p.A.: Tethis is at the forefront of developing cutting-edge workflows aimed at improving sample preparation and cellular analysis. The company’s initiatives are centered around enhancing the integration of liquid biopsy methodologies within research and clinical frameworks. Their flagship SmartBioSurface® slides leverage a proprietary process involving a nanostructured titanium coating that facilitates efficient cell adhesion, thus opening new doors for imaging, single-cell analysis, and multiplex assays. Tethis' See.d® instrument further standardizes blood sample preparation, thereby ensuring high-quality retention of samples and enabling AI-driven, comprehensive analyses of suspension cells, including rare tumor cells. Operated from Milan, Italy, Tethis continues to expand its contributions to life sciences and diagnostics, focusing on the intersection of technology and biological research. SmartBioSurface® slides and the See.d® instrument are designated for RESEARCH USE ONLY and are not approved for diagnostic use.
The focus on cell adhesion has become increasingly critical, especially when it comes to the analysis of suspension-derived samples such as Peripheral Blood Mononuclear Cells (PBMCs) and circulating tumor cells. Conventional imaging methods often struggle to maintain the integrity of these cells during repeated cycles of washing, staining, and imaging due to their lack of inherent anchorage to sample carriers. Tethis' SmartBioSurface slides are coated with a unique nanostructured titanium dioxide layer that mimics the extracellular matrix, significantly enhancing the immobilization of non-adherent cells while preserving their assay performance.
The application note showcases a rigorous study conducted using cryopreserved human PBMCs as representative samples. The findings from the study highlight several impressive outcomes:
1. Enhanced Cell Attachment: The initial attachment of PBMCs to the SmartBioSurface slides was more than double that of standard charged glass controls. This suggests that Tethis' innovative technology can provide a more effective platform for cell analysis.
2. Minimal Cell Loss: Throughout the multi-cycle imaging process, the observed cell loss averaged only about 0.14% per cycle. Remarkably, after ten imaging cycles, approximately 98.5% of cells remained intact. Additionally, no cell loss was detected after two weeks of storage at 4°C following fixation, demonstrating the slides' capacity for long-term sample stability.
3. Consistent Marker Performance: The slides exhibited reliable performance across a complex 15-plex immune panel, with all markers surpassing the established signal-to-noise threshold necessary for dependable detection and classification.
Given that samples derived from suspensions play an increasingly crucial role in spatial tissue profiling and liquid biopsies, Tethis’ SmartBioSurface slides are set to become a game-changer. They provide a stable two-dimensional layer for capturing suspension-derived cells, making them instrumental in advancing high-plex analysis of these cells, which can often be challenging due to their non-adherent nature. Tethis CEO Holger Neecke emphasized that this gentle adhesion technique plays a vital role in preserving cellular morphology and the intricate structures of suspension cells. This breakthrough paves the way for enhanced studies on rare cells and intricate immune-cell interactions, potentially unlocking new insights into various health conditions and treatments.
Furthermore, the application note characterizes SmartBioSurface slides as a scalable solution for sample preparation in high-plex biomarker profiling of suspension-derived cells. The implications of this technology are vast, with promising applications in immuno-oncology, infectious disease research, and innovative translational studies. For researchers interested in utilizing these advancements, Tethis has made the application note available for download at tethis-lab.com/smartbiosurface.
About Tethis S.p.A.: Tethis is at the forefront of developing cutting-edge workflows aimed at improving sample preparation and cellular analysis. The company’s initiatives are centered around enhancing the integration of liquid biopsy methodologies within research and clinical frameworks. Their flagship SmartBioSurface® slides leverage a proprietary process involving a nanostructured titanium coating that facilitates efficient cell adhesion, thus opening new doors for imaging, single-cell analysis, and multiplex assays. Tethis' See.d® instrument further standardizes blood sample preparation, thereby ensuring high-quality retention of samples and enabling AI-driven, comprehensive analyses of suspension cells, including rare tumor cells. Operated from Milan, Italy, Tethis continues to expand its contributions to life sciences and diagnostics, focusing on the intersection of technology and biological research. SmartBioSurface® slides and the See.d® instrument are designated for RESEARCH USE ONLY and are not approved for diagnostic use.
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