Advancements in RNA Sequencing from Lexogen
In a groundbreaking development for RNA research, Lexogen has unveiled the
miRVEL Discovery Small RNA-Seq Library Prep Kit. This innovative tool is specifically tailored to facilitate the discovery of small RNAs (sRNA) and is a game-changer in the field of biomarker studies. With a focus on low-input biofluid samples such as blood and plasma, the miRVEL Discovery kit is designed to overcome common challenges faced during RNA sequencing.
The Importance of Small RNAs in Diagnostics
Small RNAs found in biofluids can serve as powerful diagnostic and prognostic biomarkers for various diseases, including cancers and neurological disorders. However, traditional methods often struggle to isolate these small RNAs due to the presence of other abundant RNA species in biofluid samples. The miRVEL Discovery Kit addresses this critical issue by suppressing the incorporation of
hY4 Y RNA, allowing the sequencing of desired small RNAs while effectively freeing up sequencing space. This optimization leads to deeper and more precise insights into complex transcriptomic profiles, thereby enhancing the overall efficacy of RNA-Seq experiments.
Unmatched Precision with UMIs and Optimized Indexing
In addition to solving sample-specific challenges, the miRVEL Discovery kit incorporates
Unique Molecular Identifiers (UMIs), which play a vital role in generating accurate and unbiased quantification of RNA. By eliminating PCR duplicates, researchers can trust that their quantification results are reflective of the true biological conditions.Paired with
10 nt unique dual indices (UDIs), the kit ensures precise read assignments while reducing the chances of index hopping. This enables reliable multiplexing of up to 120 samples, making the miRVEL Discovery kit an essential asset for busy laboratories managing high-throughput studies.
Streamlined Workflow for Efficiency
One of the standout features of the miRVEL Discovery kit is its
gel-free protocol. Researchers can complete the entire library preparation for sequencing in less than seven hours. This efficiency stems from the use of modified oligonucleotides that prevent adapter dimer formation, eliminating the laborious gel-size selection step seen in many traditional methodologies. Consequently, researchers can prepare their samples more rapidly, facilitating timely downstream analyses and enabling quicker turnarounds on critical research timelines.
Exceptional Performance on Diverse Sample Types
Extensive testing reveals that the miRVEL Discovery Small RNA-Seq Library Prep Kit excels with low-input biofluid samples, demonstrating remarkable data correlation across various concentrations. Additionally, it showcases impressive reproducibility among replicates, even with minimal inputs, ensuring that results are reliable and valid. Furthermore, it outshines competitor products by delivering a higher rate of reads mapping to microRNAs (miRNAs) and detecting an increased number of miRNAs in both plasma and blood samples, confirming its superiority in the realm of small RNA sequencing.
Conclusion: Availability and Implications for Research
The miRVEL Discovery Small RNA-Seq Library Prep Kit is now available for order, representing a significant leap forward for researchers focusing on sRNA and the quest for innovative biomarker discovery. With its advanced design and user-centric features, Lexogen continues to establish itself as a leader in RNA sequencing technologies. For those interested in elevating their research capabilities, this new library prep kit is poised to make a notable impact in the field of transcriptomics.
For more information about the miRVEL Discovery kit, visit Lexogen's official website at
www.lexogen.com and discover how this innovative tool can enhance your research.