RNAConnect Introduces Revolutionary UltraMarathonRT for RNA Sequencing

RNAConnect Unveils UltraMarathonRT cDNA Synthesis and Amplification Kit

RNAConnect, recognized for its innovations in molecular biology, has officially introduced the UltraMarathonRT cDNA Synthesis and Amplification Kit. This groundbreaking tool is designed to transform short-read RNA sequencing workflows, delivering heightened sensitivity and extensive detection capabilities even for challenging transcripts.

Dr. Jason Underwood, Vice President of Technology Development at RNAConnect, emphasized the significance of this advancement: "UltraMarathonRT marks a major advancement for RNA sequencing. By leveraging the capabilities of uMRT, researchers can now explore RNA species and transcriptional complexities that were previously challenging to identify, particularly rare isoforms and long noncoding RNAs. This opens up new pathways for biomarker discovery and fundamental research."

Addressing Common Limitations

Despite the advancements in RNA sequencing technologies, researchers face limitations primarily due to the challenges associated with creating cDNA inputs that accurately represent the transcriptomic diversity of RNA samples. Traditional retroviral reverse transcriptases (RTs) often encounter 'distributive' behavior, whereby they frequently detach from RNA transcripts, subsequently struggling to synthesize through complex or lengthy sequences. Because of this, extensive segments of RNAs are often left uncaptured in cDNA and eventually do not make it into sequencing libraries.

The UltraMarathonRT kit tackles these issues head-on. It utilizes a group II intron encoded RT that is built for ultraprocessivity, thanks to its intrinsic helicase activity. This design allows it to faithfully replicate RNA from end to end in a single pass, which can be performed at ambient temperatures.

Enhancements Over Existing RT Kits

The UltraMarathonRT cDNA Synthesis and Amplification Kit stands out when compared to leading reverse transcriptase kits. Here are some of the key enhancements it offers:

1. Improved Detection of Genes and Isoforms: UltraMarathonRT significantly boosts the reverse transcription of low-abundance and structurally intricate RNAs, ensuring that transcripts and retained introns which often evade detection with traditional retroviral RT kits are now captured effectively.
2. Superior Quantification: With its ultraprocessive and high-fidelity reverse transcriptase, UltraMarathonRT guarantees full-length cDNA synthesis, leading to improved accuracy in quantifying longer transcripts.
3. Seamless Integration: The kit is designed to fit effortlessly into existing short-read RNA-seq workflows, compatible with common Illumina-style platforms and library preparation methods, thus serving as a versatile solution for researchers in differential expression studies, biomarker discovery, intron retention analysis, and splice isoform mapping.

Currently, the UltraMarathonRT is available for commercial purchase in major markets globally through www.rnaconnect.com. The kits come in various sizes, suitable for everything from pilot projects to large-scale high-throughput workflows. Additionally, customized OEM and bulk packaging solutions are accessible for core facility users and those requiring large volumes.

About RNAConnect

RNAConnect is dedicated to advancing life sciences through innovative RNA analysis solutions. Their goal is to empower researchers with tools that enhance the study of genomics, transcriptomics, and molecular diagnostics. The company's products facilitate discoveries across various fields, including rare diseases, immunology, oncology, and neurobiology. With a focus on sensitivity, accuracy, and reliability, RNAConnect continues to be at the forefront of RNA research tools.