#USA #New England Biolabs #Ipswich #Monarch #cfRNA

New England Biolabs Launches Monarch cfRNA Extraction Kit

New England Biolabs (NEB) has recently announced the launch of the Monarch Mag Cell-free RNA (cfRNA) Extraction Kit, aimed at researchers who require efficient and reliable isolation of cell-free RNA from various biofluids. This innovative kit is particularly useful for isolating low-abundance cfRNA and exome-associated RNA from sources like plasma, serum, urine, and cerebrospinal fluid.

Traditionally, cell-free DNA (cfDNA) has been the primary analyte used in liquid biopsies, but recent findings underscore the importance of cfRNA, which can provide insights that cfDNA cannot. According to Anagha Kadam, Applications and Product Development Scientist II at NEB, cfRNA can reveal the origins of signals in tissues and allow researchers to track pathological changes over time. With this new kit, researchers can expect consistent recovery of low-abundance cfRNA while effectively removing DNA contamination, enabling more accurate assessments of this critical but often understudied analyte.

Overcoming Technical Challenges

The Monarch cfRNA Extraction Kit addresses two common challenges faced during total circulating RNA extraction: DNA carryover and the loss of small RNAs. Utilizing chaotropic salt-based chemistry paired with magnetic silica beads, this kit offers a gentle yet efficient alternative to conventional phenol-based methods. It incorporates lyophilized DNase, crucial for minimizing contaminating DNA that could skew downstream results, ultimately providing a clearer cfRNA signal across diverse sizes, including long non-coding RNAs, small RNAs, and microRNAs.

By lyophilizing temperature-sensitive reagents, NEB has created a kit that can be shipped ambiently, streamlining the operational processes for researchers. This approach aligns with NEB's commitment to sustainability within its product portfolio. Additionally, the kit's flexible sizing and automation compatibility allow for scalable reagent usage tailored to researchers' specific needs.

A Hands-On Approach to Research Support

According to James Deng, Senior Product Marketing Manager for NEB, researchers often encounter numerous challenges throughout their workflows, especially with emerging applications related to both cfDNA and cfRNA extraction. NEB's comprehensive support spans the entirety of extraction processes, downstream sequencing, and amplification, empowering researchers with technical guidance that is both holistic and innovative.

Furthermore, the Monarch cfRNA Extraction Kit complements NEB's existing products, including the NEBNext UltraExpress® Library Prep Kit and the Low Bias Small RNA Library Prep Kit, crucial for conducting total transcriptomic analysis and small RNA profiling, respectively. These synergies enhance the practical applications of the cfRNA Extraction Kit in diverse research settings.

Conclusion

With the Monarch Mag Cell-free RNA Extraction Kit, New England Biolabs continues to empower researchers in the field of molecular biology. By providing streamlined workflows and robust technical support, NEB reaffirms its commitment to driving advancements in life sciences. To gain deeper insights and access performance data related to the Monarch cfRNA Extraction Kit, interested parties can visit NEB's official website.

About New England Biolabs

For over five decades, New England Biolabs has been at the forefront of molecular biology research, developing innovative products tailored for scientific discovery. NEB is dedicated to supporting customers in both academia and industry, while also focusing on improving the well-being of its employees and surrounding communities. With a global presence and a broad distribution network, NEB continues to drive progress across the scientific landscape. For more information, visit www.neb.com.