MOBILion Systems Showcases Revolutionary Proteomics Advances at HUPO 2025 Congress

MOBILion Systems, a frontrunner in separation science and analytical innovations, is gearing up for an exciting presentation at the 24th Annual Human Proteome Organization (HUPO) World Congress. This prestigious event will take place from November 9 to 13, 2025, in Toronto, Canada. The company will highlight its pioneering technology known as Parallel Accumulation Mobility Aligned Fragmentation (PAMAF™), which significantly enhances mass spectrometry (MS) sensitivity and throughput for proteomics and multi-omics applications.

In the realm of proteomics, the focus has shifted to the analysis of post-translational modifications (PTMs) and distinct proteoforms. As medicine advances, these elements are becoming critical in drug development and clinical diagnostics. Therefore, robust technologies capable of deepening detection and boosting confidence in results are transforming the landscape of biological research.

MOBILion's PAMAF technology stands out because it utilizes High-Resolution Ion Mobility (HRIM), achieving near-total ion utilization while ensuring spectral generation rates surpassing 500 Hz. This breakthrough represents a significant leap forward, as it replaces the traditionally slower quadrupole filtering method that has hindered detection efficiency in mass spectrometry. As a result, PAMAF not only maximizes ion detection but also amplifies MS/MS signal intensity and speeds up data acquisition, making it a revolutionary tool for researchers.

Dr. Melissa Sherman, the CEO of MOBILion Systems, emphasizes the importance of PAMAF technology in proteomics research. She states, "In a field where even modest improvements are seen as significant, PAMAF exhibits improvements ranging from 300 to 2,000% in fragment ion intensity, dramatically boosting identification rates. This change will empower researchers to identify thousands of additional proteins and PTMs from reduced sample sizes, unlocking new opportunities in proteomics and therapeutic development."

At the HUPO 2025 event, Chief Technology Officer Daniel DeBord, Ph.D., will present key benchmark data drawn from LC-HRIM-MS experiments that couple MOBILion's innovative MOBIE® HRIM module with an established QTOF platform operated in PAMAF mode. The compelling results indicate up to a 100-fold increase in fragment ion intensity, identification of 3 to 12 times more protein groups, and 43 to 53% enhancements in peptide counts per protein derived from cell line digests. These advancements are particularly striking when utilizing low sample inputs, marking a transformative step for workflows in areas like single-cell proteomics and quantitative PTM analyses.

In conclusion, Dr. DeBord remarks on the vital role of improved ion utilization in mass spectrometry performance. By eliminating the quadrupole filtering process and synchronizing fragmentation with ion mobility, PAMAF opens the door to more exhaustive and unbiased investigations across diverse biological samples. This advancement is crucial in the pursuit of complete proteome and proteoform coverage, and aligns with MOBILion's mission to enhance the depth, precision, and rapidity of proteomics measurements through revolutionary innovations.

MOBILion's participation in HUPO 2025 not only signifies their commitment to advancing proteomics technology but also reflects their dedication to supporting the scientific community in uncovering the mysteries of life at a molecular level. As they continue to develop high-performance analytical instruments that blend state-of-the-art technology with practical application, MOBILion Systems is poised to make a profound impact on the future of proteomics and related fields.